In Silico Validation Studies of Cyanobacterial Bioactive Compounds Against Α-amylase and Α-glucosidase Markers in Type 2 Diabetes Mellitus
RESEARCH ARTICLE

In Silico Validation Studies of Cyanobacterial Bioactive Compounds Against Α-amylase and Α-glucosidase Markers in Type 2 Diabetes Mellitus

Suhail Ahmad1 Salman Akhtar1 , * Open Modal Alvina Farooqui1 , * Open Modal
Authors Info & Affiliations
The Open Bioinformatics Journal 11 Mar 2024 RESEARCH ARTICLE DOI: 10.2174/0118750362284095240301035202

Abstract

Aim

The study aims to assess the binding efficiency of cyanobacterial compounds against key Type 2 Diabetes Mellitus (T2DM) targets, α-amylase and α-glucosidase, using an in-silico approach. Additionally, it aims to design drugs with minimal adverse effects or no toxicity to inhibit the complications and help in the management of T2DM.

Methods

Twenty-five (25) cyanobacterial bioactive compounds were sourced from various cyanobacterial strains via the PubChem database. The three-dimensional structures of the target proteins, α-amylase (1KB3) and α-glucosidase (1QOX) were obtained from RCSB PDB and visualized using Discovery Studio Visualizer 3.0. Molecular docking was performed using AutoDock 4.2 and Cygwin.

Results

Studies revealed that Ethyl tumonoate A, Debromoaplysiatoxin, and Scytoscalalrol exhibited higher binding interactions with α-amylase (1KB3), while Ambiguine I Isonitrile, Scytoscalalrol, and Cylindrospermopsin displayed higher binding affinities with α-glucosidase (1QOX) among the tested cyanobacterial bioactive compounds. These compounds exhibited greater binding affinities compared to synthetic drugs like metformin (-7.66 kcal/mol) and acarbose (-8.86 kcal/mol).

Conclusion

Our findings suggest that cyanobacterial bioactive compounds, particularly Ethyl tumonoate A, Ambiguine I Isonitrile, Cylindrospermopsin, and Scytoscalalrol, possess potential binding affinities with T2DM-related targets, making them promising lead compounds for the development of novel drugs with fewer side effects for the management of T2DM and its associated complications.

Keywords: T2DM, Diabetes markers, Cyanobacterial bioactive compounds, Bioinformatics, Molecular docking, α-amylase, α-glucosidase.

1. INTRODUCTION

A chronic condition of the metabolism of carbo- hydrates, lipids, and proteins known as diabetes mellitus (DM) is characterized by elevated postprandial and fasting blood glucose levels (hyperglycemia). Diabetes mellitus is caused by either impaired insulin action or insufficient insulin production [1]. Abnormal metabolism of lipids and proteins is a key provider to illness and death in many developing and developed countries, including China, India United States and several other countries. Diabetes mellitus has significant statistical implications. According to current data published by the World Health Organization (WHO) up to March 2013, the incidence of diabetes cases has increased from 153 million patients in 1980 to 347 million persons globally with diabetes mellitus in 2008. (DM). Furthermore, it is estimated that by 2025, this number will have risen to 380 million, accounting for 7.1 percent of the world's adult population [2] and it is supposed to be 500 million up to 2030 [3]. The selection of proper preventive measures is necessary to reduce the disease burden on the health and economy. Although several synthetic drugs exist for the treatment of diabetes mellitus, none of these medications are low-priced or completely effective. Furthermore, prolonged use of these drugs may produce undesirable side effects [4]. As a result, diabetes mellitus is a serious public well-being issue that affects millions of people in high, middle and low-income nations [5], and abnormal metabolism of proteins and lipids is a major contributor to morbidity and mortality in many developed and developing countries, including the United States and India. The insulin functions, including secretion, site of action and action, when altered or defected, are the principal factors responsible for diabetes. The severity of diabetes is connected with the consecutive destruction of microstructures, mainly the retina, nephron, and neurons, affecting eyes, kidneys and nerves. The disease is also associated with several cardiovascular diseases and related complications [6, 7]. Apart from that, oxidative stress also plays a vital role in the development of diabetes mellitus and its complications [4]. The selection of proper preventive measures is necessary to reduce the disease burden on the health and economy. One of the main salivary proteins, alpha-amylase, has been suggested as a sensitive, non-invasive biomarker whose primary function is the enzymatic digestion of carbohydrates by the hydrolysis of starch to sugar and maltose [8]. Treatment for abnormalities in carbohydrate uptake, including diabetes, obesity, dental caries, and periodontal illnesses, includes inhibition of the enzyme α-amylase, which is involved in the digestion of starch and glycogen. It serves as a marker for the diagnosis of diabetes mellitus when it is found to be significantly higher in diabetics than in non-diabetics, leading to excessive glucose production. A few studies have also suggested that reducing the absorption of complex carbs while using a salivary α-amylase inhibitor may help manage blood sugar levels [8]. Therefore, there is a need for additional research to support the earlier results. Thus, there is a need for more studies supporting the previous studies. Alpha-glucosidase was 1.5 times overexpressed in non-insulin-dependent diabetes mellitus patients, which increased postprandial glucose levels, according to reports [9]. The body absorbs monosaccharides and polysaccharides produced by α-amylase and α-glucosidase at varying rates, with monosaccharide components being absorbed very rapidly. It follows that inhibiting α-amylase and α-glucosidase action can delay the release of glucose from complex carbs, modifying the start of postprandial hyperglycemia, making it an excellent target for the treatment or management of type 2 diabetes mellitus [10]. Acarbose, a pseudo-carbohydrate derived from actinomycetes, is the α-glucosidase inhibitor that is most frequently administered [11]. In the present years, a quantity of bioactive compounds and their analogues from cyanobacteria with promising antibacterial, antiviral, anticancer, antifungal, antioxidant, anti-inflammatory, antidiabetic and anthel- mintic potentials have been explored [12].

Novel development for medications in all major disease categories can be found in natural resources, which constitute a significant source. Recently, the main research focus has been on generating novel medications using cyanobacteria [13]. They are present throughout the world, from the Antarctic to hot springs, deserts, soils, salt lakes, and brine water [14]. They serve as models for photosynthetic prokaryotes for the study of numerous metabolites produced by these organisms under such conditions due to their capacity to develop in a variety of ecological conditions, including light, temperature, salinity, alkalinity, and pollution [15-17]. According to studies [18, 19], the biologically active chemicals extracted from cyanobacteria exhibit antibacterial, antiviral, antifungal, enzyme inhibitory, immune-stimulant, cytotoxic, and anti-plasmodium effects. Additionally, cyanobacteria are a good source of additional vital compounds such as vitamins, amino acids, and fatty acids [20]. Biochemically active chemicals have been produced by cyanobacteria like Microcystis, Anabaena, Nostoc, Spirulina sp. and, Oscillatoriaetc [21]. The secondary metabolites produced by cyanobacteria are novel bioactive constituents used in the production of pharmaceuticals and vital chemicals for agriculture and various fields. It has been discovered that cyanobacterial lipopeptides are intriguing, biochemically active constituents. An enormous 85% of them are bioactive, including cytotoxic (41%), anticancerous (13%), antibiotics (12%), enzyme inhibitors (8%), antiviral (4%) and antifungal (4%) among their prominent properties [22]. The outstanding activity (18%) includes UV-absorbing properties that can be usable as sunscreens, herbicides, antimalarial, antimycotic, antimitotic, anti microalgal and activity that promotes cell differentiation [22].

Through the use of an in-silico approach, it is possible to identify lead compounds with the best binding energies and affinities for further study. These methods offer insightful information on the molecular interactions between bioactive constituents and target enzymes [23]. This study aims to investigate the prominent role of bioactive compounds derived from cyanobacteria in the treatment of type 2 diabetes mellitus, concentrating on how they interact with the enzymes α-amylase and α-glucosidase. The use of In-silico methods is to examine the affinities and binding energies of the aforementioned cyanobacterial bioactive compounds to the active sites of these enzymes. The research will clarify the inhibitory capability of compounds from cyanobacteria and their ability to control glucose release and carbohydrate digestion, helping to manage type 2 diabetes mellitus [24].

2. MATERIALS AND METHODS

2.1. Software and Tools

The recent study was executed by using bioinformatics tools and software like Auto Dock 4.2, Cygwin, and Discovery Studio 3.0.Operating systems that support 64-bit Windows 10 and 32-bit Windows 7 include biological databases like PubChem (http://pubchem.ncbi.nlm.nih. gov/), Protein Data Bank (www.rcsb.org/), Molsoft (https://www.molsoft.com/) and Pre ADMET (https://preadmet.webservice. bmdrc.org/).

2.2. Retrieval and Preparation of Targeted Protein Structures

The main source for proteins having experimentally verified structures is the RCSB PDB (Research Collaboratory for Structural Bioinformatics, Protein Data Bank). It contains a large number of 3-dimensional structures that were discovered by techniques like NMR, X-ray crystallography, etc. The PDB was used to retrieve the targeted crystal structure of the proteins PDB ID: 1Kb3 (α- amylase) and 1qOX (α-glucosidase) shown in Figs. (1 and 2) from RCSB PDB (https://www.rcsb.org/.). The required hydrogen atoms were added after all water molecules, and crystallographic substructures from the specified protein were taken out to nullify the overall charges for molecular docking purposes. Furthermore, Discovery Studio Visualizer 3.0 was used to visualize the final structure, which shows the 3-D structure of selected proteins.

Fig. (1). 3-D structure of α- amylase (PDB ID: 1Kb3).
Fig. (2). 3-D structure of α-glucosidase (PDB ID: 1qOX).

2.3. Ligand Library Preparation

All filtered derivatives that were retrieved in 3D sdf format from the PubChem database are included in the compound library. The library includes several hundred compounds that have been screened using Lipinski's rule of five and ADME filters. The drug molecules or chemical compounds' absorption, distribution, metabolism, excretion, and toxicity inside the animal body are represented by the ADME and toxicity profile. The blood-brain barrier, CaCo2, and AMES Toxicity are among the characteristics that make up the ADME profile. The network of blood arteries and tissues known as the blood-brain barrier protects the brain from hazardous constituents. The drug's enormous concentration will make it extremely difficult and even impossible for the human body to survive if it crosses the BBB. Human intestinal absorption (HIA) is a characteristic that shows that medications taken orally are absorbed into the bloodstream through the gastrointestinal tract. For a medication to be therapeutically effective inside the human body, it must have an HIA characteristic. The Ames test is a frequently used method for assessing a substance's genotoxicity by measuring its potential harm to human health through the identification of mutagenic characteristics. This test is defined by its exceptional repeatability and limitless sensitivity. We found 25 compounds with good ADME and toxicity characteristics after filtering.

2.4. Screening and Retrieval of Cyanobacterial and Control Compounds with Chemical Structures

A public chemical database called PubChem (https://pubchem.ncbi.nlm.nih.gov) was developed by the National Library of Medicine (NLM), a division of the National Institutes of Health (NIH) in the United States [25 Hundreds of bioactive compounds from different cyanobacterial strains selected by the literature survey were downloaded from PubChem database for the molecular docking studies and their chemical structures. Selected bioactive compounds filtered based on Lipinski’s rule of five (R05) and ADMET profile. Twenty-five cyanobacterial bioactive compounds selected out of 100, namely are - Acarbose (CID: 6918537), Metformin (CID: 4091), Ambiguine B Isonitrile (CID: 16109784), Ambiguine H Isonitrile (CID: 16069590), Ambigol A (CID: 475341), Ambigol B (CID: 475342), Anatoxin-a (CID: 3034748), Aplysiatoxin (CID: 46173823), Carazostatin (CID: 130857), Carbamydocyclophane E (CID: 16216032), Carbamy- docyclophane J (CID: 16216032), Curacin A (CID: 5281967), Curacin D (CID: 5281967), Debromoplysiatoxin (CID: 5352033), Nostocarboline (CID: 5326150), Ethyl Tumonoate A (CID: 53493313), Nostocine A (CID: 10749358), Nostocyclyne A (CID: 10088786), Radiosumin (CID: 102506248), Rutin (CID: 5280805), Tanikolide (CID: 5276592), Venturamide B (CID: 16115401) Scytoscalarol (CID: 44605340) and also other compounds with chemical structures retrieved from ‘PubChem’ database are shown in Table 1.

Table 1.
Chemical structures of antidiabetic drugs and cyanobacterial bioactive compounds with PubChem No. and their source.
S.No. Compounds Name PubChem No. Source of Compounds Compounds Structure
1 METFORMIN (CONTROL) 4091 Galega officinalis
2 ACARBOSE
(CONTROL)
41774 Synthetic drug
3 Ambiguine I isonitrile 16109784 Fischerella sp.
4 Ambiguine H isonitrile 16069590 Fischerella sp.
5 Ambigol A 475341 Fischerella ambigua
6 Ambigol B 475342 Fischerella ambigua
7 Anatoxin-a 431734 Anabaena circinalis
8 Aplysiatoxin 21672114 Geitlerinema
9 Carazostatin 130857 Hyella caespitose
10 Carbamidocyclophane E 16216032 Nostoc sp.
11 Carbamidocyclophane J 122206459 Nostoc sp.
12 Curacin D 10546404 Lyngbya sp.
13 Curacin A 5281967 Lyngbya sp.
14 Cylindrospermopsin 42628600 Cylindrospermopsis sp.
15 Debromoaplysiatoxin 5352033 Lyngbya majuscule
16 Ethyl Tumonoate A 53493313 Oscillatoria margaritifera
17 Nostocarboline 5326150 Nostoc sp.
18 Nostocine A 10749358 Nostoc spongiae forme
19 Nostocycline A 10088786 Nostoc sp.
20 Radiosumin 10812638 Plectonema radiosum
21 Rutin 5280805 Gracilaria dendroides
22 Scytoscalalrol 44605340 Scytonema pseudohofmanni
23 Tanikolide 5276592 Lyngbya majuscula
24 Tjipanazole J 15705741 Tolypothrix tjipanasensis
25 Toyocamycin 11824 Tolypothrix tenuis
26 Tubercidin 6245 Plectonema radiosum
27 Venturamide B 16115401 Oscillatoria sp.
 

2.5. Prediction of ADME and Toxicity Profile

All 100 of the resulting compounds were subsequently approved to go through ADME and toxicity filtration after the Lipinski filter had been applied. All 100 cyanobacterial compounds' ADMET properties were predicted using the preADMET tool, and only candidates that favoured the values of the BBB, CaCo2, HIA, and AMES toxicity and Mouse/Rat carcinogenicity parameters were chosen. Out of 100 compounds, 25 were found to have useful ADMET profiles after the ADMET screening. The same preADMET web server was used to evaluate the toxicity profile of the 25 compounds that were chosen.

2.6. Molecular Docking using AutoDock 4.2 and Cygwin

The bioinformatics tools AutoDock 4.2 and Cygwin were used to evaluate and identify the lowest free binding energy of the selected cyanobacterial compounds along with 2 reference compounds against the targeted protein α- amylase (1Kb3) and α-glucosidase (1qOX) which is associated with type 2 diabetes mellitus.

3. RESULTS AND DISCUSSION

3.1. Drug-likeness Properties of Cyanobacterial Compounds

The main purpose of Lipinski's rule of five, commonly referred to as the thumb rule, is to assess the drug-likeness of a chemical compound in order to establish whether or not a compound with a particular pharmacological activity is orally active in humans. For a molecule to function as a medication and demonstrate good permeability through cell membranes, the log P value must be lower than 5. Other criteria for this rule state that the number of hydrogen bond acceptors must be ≤ 10 (The sum of Os and Ns), the number of hydrogen bond donors must be ≤ 5 (The sum of OHs and NHs), and molecular weight should be <500Da. If the number of violations are equal to 1 or no violation, it states that the compound can simply bind to the target receptor [26]. Drugs are excluded from further screening if more than two parameters are out of the standard range [27]. Out of 100 screened compounds from cyanobacteria, only 25 compounds were filtered through Lipinski’s Rule of five and were taken further for ADMET profiling. The list of filtered compounds is given in Table 2.

3.2. ADMET Evaluation of Filtered Compounds

According to Table 3, 25 filtered compounds from Lipinski's rule of five were used in the ADMET investigation. Studies show that poor pharmacokinetics and toxicity are the main reasons for expensive late-stage drug development failures, and it is now generally recognized that these factors should be taken into account as early as feasible in the drug discovery process. The number of compounds for which preliminary data on absorption, distribution, metabolism, excretion (ADME), and toxicity (T) are required has greatly expanded as a result of combinatorial chemistry and high-throughput screening [27]. PreADMET, a tool that is accessible online (https://preadmet.webservice.bmdrc.org), evaluates ADME attributes.

Table 2.
Drug likeness properties of filtered bioactive compounds from cyanobacteria along with control drug.
S. No. Name of the Compounds Drug Likeness Properties Drug- Likeness Score
LogP Molecular Weight Hydrogen Bond Donor Hydrogen Bond Acceptor
A METFORMIN (CONTROL) -1.00 129.10 5 2 -0.82
B ACARBOSE
(CONTROL)
-4.58 645.25 14 19 0.40
1 Ambiguine I Isonitrile 6.19 402.50 2 2 -1.1
2 Ambiguine H Isonitrile 7.58 372.26 1 1 -0.78
3 Ambigol A 8.36 485.00 2 3 -0.14
4 Ambigol B 8.16 485.00 1 3 -0.48
5 Anatoxin-a 1.53 165.23 1 1 -1.42
6 Aplysiatoxin 4.93 670.24 3 10 0.32
7 Carazostatin 6.83 295.40 2 1 -0.41
8 Carbamydocyclophane E 8.71 670.90 8 8 0.82
9 Carbamydocyclophane J 8.71 670.90 8 8 0.82
10 Curacin A 6.51 373.60 0 3 0.35
11 Curacin D 6.51 373.60 0 3 0.35
12 Debromoplysiatoxin 4.33 592.70 3 10 0.47
13 Nostocarboline 3.36 217.67 1 0 -0.94
14 Ethyl Tumonoate A 5.08 367.50 1 4 -0.60
15 Nostocine A -0.92 151.13 1 4 -1.16
16 Nostocyclyne A 8.36 342.50 2 2 -0.08
17 Radiosumin -1.95 432.50 6 6 -0.21
18 Rutin -1.55 610.50 10 16 0.91
19 Scytoscalalrol 4.83 415.7 5 2 -0.47
20 Tanikolide 5.34 284.40 1 3 -0.95
21 Tjipanazole J 5.94 290.7 2 0 -1.31
22 Tolyporphin 4.08 742.8 4 12 0.02
23 Toyocamycin -1.02 291.26 5 7 0.53
24 Tubercidin -0.92 266.25 5 6 0.54
25 Venturamide B 1.75 518.60 4 10 -0.58
Abbreviation: Log P- partition coefficient.
Table 3.
ADMET profiling of filtered cyanobacterial bioactive compounds using Pre ADMET server.
S. No. Name of The Compound BBB HIA Caco2 AMES Toxicity Carcinogenicity
Mouse/rat
1 Ambiguine I Isonitrile 7.45316** 92.849612 23.9099 Mutagen Positive/ Positive
2 Ambiguine H isonitriles 16.5872 97.046579 48.6152 Mutagen Positive/ Negative
3 Ambigol A 13.4989 95.546983 46.5271 Mutagen Negative/Positive
4 Ambigol B 14.9377 96.842893 56.983 Mutagen Negative/Positive
5 Anatoxin-a 0.612474 95.398255 30.5172 Mutagen Positive/ Negative
6 Aplysiatoxin 0.62309 93.927517 21.7641 Non-Mutagen Negative/Negative
7 Carazostatin 15.6199 93.267025 45.6263 Mutagen Negative/Negative
8 Carbamidocyclophane E 6.6225 78.754511 20.4097 Mutagen Negative/Negative
9 Carbamidocyclophane J 6.54959 85.988582 21.0478 Mutagen Negative/Negative
10 Curacin D 3.20083 97.506753 31.6657 Mutagen Positive/ Negative
11 Curacin A 5.7525 97.546286 35.2846 Non-Mutagen Positive/ Negative
12 Cylindrospermopsin 0.0412124 69.533771 21.0569 Mutagen Negative/Positive
13 Debromoaplysiatoxin 1.92282 96.111782 33.1237 Non-Mutagen Negative/Negative
14 Ethyl Tumonoate A 0.239313 90.065359 24.9041 Mutagen Positive/Negative
15 Nostocarboline 5.18456 97.910575 16.7822 Mutagen Positive/Negative
16 Nostocine A 0.114421 78.765338 11.6462 Mutagen Positive/Positive
17 Nostocycline A 18.0699 93.610401 47.7531 Mutagen Negative/Negative
18 Radiosumin 0.0598323 63.980144 20.8546 Mutagen Positive/Positive
19 Rutin 0.0285642 2.861176 7.91267 Non-Mutagen Negative/Negative
20 Scytoscalarol 0.75716 92.010946 21.0215 Mutagen Negative/ Positive
21 Tanikolide 7.06301 94.877159 46.7781 Non-Mutagen Negative/Negative
22 Tolyporphin J 0.067607 82.169793 0.808384 Non-Mutagen Positive/Positive
23 Toyocamycin 0.112285 46.547342 5.36832 Mutagen Negative/ Positive
24 Tubercidin 0.360937 65.797783 5.22828 Mutagen Negative/Negative
25 Venturamide B 0.0561416 56.779147 9.19956 Mutagen Negative/ Positive
Abbreviations: BBB- Blood brain barrier; HIA-Human intestinal absorption; Caco-2- heterogenous human epithelial colorectal adenocarcinoma; M- mutagen; C-carcinogen (rat/mouse).

A total of 25 ligands from Cyanobacteria were selected out of 100 for the molecular docking investigation. After molecular docking investigation of selected ligands out of 25 top 3 compounds, namely, Ethyl Tumonoate A, Debromoaplysiatoxin and Scytoscalarol, showed highly significant interaction with α-amylase listed in Table 4 and also out of 25 ligands top 3 compounds namely, Ambiguine I isonitrile, Cylindrospermopsin and Debromoaplysiatoxin showed excellent binding interaction with α-glucosidase listed in Tables 5 and 6.

The molecular docking interaction results reveal the free binding energy of α-amylase was found with Metformin (reference compound) concluded 10 amino acid residues, namely, Ile 235, Glu 233, Ala 193, Asp 197, Asp 96, Tyr 62, Trp 58, His 299, Asp 300, His 305 (Fig. 3); with Ehtyl Tumunoate A concluded 10 amino acid residues- Glu 171, Asp 206, Gly 205, Pro 204, Trp 203, Asn 137, Lys 140, Asp 159, Arg 158, Thr 155 (Fig. 4); with Debromoaplysiatoxin concluded 07 amino acid residue- Pro 241, Lys 243, Ala 154, Asp 246, Gly 205, Trp 203, Arg 158 (Fig. 5); with Scytoscalarol concluded 05 amino acid residues- Thr 155, Asp 159, Lys 140, Trp 203, Glu 171 (Fig. 6). The values of free binding energy for Metformin with α-amylase, Ehtyl Tumunoate A with α-amylase, and Scytoscalarol with α-amylase docking scores originated were –7.66, –9.28, –7.97, -7.14 kcal/mol respectively, although the conforming values of inhibition constant (Ki) were expected to be 2.43, 0.5765, 1.43, 5.80 µM separately listed in Table 5.

Figs. (3-6): Docked structure of selected top three leads and Metformin, a control drug, with the active site residues of α-amylase.

Table 4.
Binding energy of docked cyanobacterial bioactive compounds and metformin (control) with protein (α-amylase).
S. No. Name of Compounds Free Binding Energy
Kcal/mol
Inhibition Constant
Ki (μM)
0 METFORMIN (Control) -7.66 2.43
1 Ethyl Tumonoate A -9.28 0.15765
2 Debromoaplysiatoxin -7.97 1.43
3 Scytoscalarol -7.14 5.80
4 Ambigol A -4.93 241.67
5 Ambigol B -5.21 152.01
6 Ambiguine H isonitriles -4.95 235.76
7 Tanikolide -3.28 3910
8 Tjipanazole J -5.63 75.05
9 Tolyporphin J -6.07 35.63
10 Toyocamycin -5.27 116.27
11 Tubercidin -4.62 411.82
12 Venturamide B -5.02 209.47
13 Nostocarboline -4.45 550.91
14 Nostocine A -4.56 493.97
15 Nostocycline A -6.21 28.07
16 Radiosumin -5.2 153.42
17 Rutin -4.39 606.76
18 Carbamidocyclophane E -4.13 940.81
19 Carbamidocyclophane J -3.34 3.58
20 Curacin D -6.31 23.89
21 Curacin A -3.23 4270
22 Ambiguine B isonitrile -5.17 162.87
23 Anatoxin-a -5.00 217.36
24 Aplysiatoxin -5.49 95.04
25 Carazostatin -5.20 155.48
Table 5.
Active sites residues in the receptor of top three cyanobacterial bioactive compounds and metformin with Binding energy (receptor: α-amylase).
S. No. Name of Compounds Free Binding Energy
Kcal/mol
Interacting Residues in the Receptor
0 METFORMIN (CONTROL) -7.66 Ile 235, Glu 233, Ala 193, Asp 197, Asp 96, Tyr 62, Trp 58, His 299, Asp 300, His 305
1 Ethyl Tumonoate A -9.28 Glu 171, Asp 206, Gly 205, Pro 204, Trp 203, Asn 137, Lys 140, Asp 159, Arg 158, Thr 155
2 Debromoaplysiatoxin -7.97 Pro 241, Lys 243, Ala 154, Asp 246, Gly 205, Trp 203, Arg 158
3 Scytoscalarol -7.14 Thr 155, Asp 159, Lys 140, Trp 203, Glu 171
Table 6.
Binding energy of docked cyanobacterial bioactive compounds and acarbose (control) with protein (α-glucosidase).
S. No. Name of Compounds Free Binding Energy
Kcal/mol
Inhibition Constant
Ki (μM)
0 ACARBOSE (control) -8.86 0.31974
1 Ambiguine I isonitriles -9.46 0.11641
2 Scytoscalarol -7.5 3.2
3 Cylindrospermopsin -7.17 5.51
4 Rutin -2.01 33410
5 Ambiguine H isonitriles -4.97 226.79
6 Tanikolide -5.05 198.48
7 Tjipanazole J -6.18 29.62
8 Toyocamycin -6.59 14.68
9 Venturamide B -5.79 57.3
10 Debromoaplysiatoxin -4.94 241.16
11 Ethyl Tumonoate A -3.47 2870
12 Nostocarboline -4.51 497.92
13 Nostocine A -4.57 445.18
14 Nostocycline A -4.97 227.14
15 Radiosumin -4.88 267.02
16 Carbamidocyclophane J -2.42 16750
17 carbamidocyclophane c -2.42 16780
18 Ambigol A -5.82 54.22
19 Ambigol B -6.55 15.72
20 Anatoxin-a -6.10 33.65
21 Aplysiatoxin -4.52 490.20
22 Carazostatin -6.07 35.76
23 Carbamidocyclophane E -2.94 7.04
24 Curacin D -4.70 1.03
25 Curacin A -4.24 776.45
Fig. (3). Docked structure of metformin and α-amylase.
Fig. (4). Docked structure of ethyl tumonoate A and α-amylase.
Fig. (5). Docked structure of debromoaplysiatoxin and α-amylase.
Fig. (6). Docked structure of scytoscalarol and α-amylase.
Fig. (7). Docked structure of acarbose and α-glucosidase.
Fig. (8). Docked structure of ambiguine I isonitriles and α-glucosidase.

Similarly, the molecular docking interaction revealed that the free binding energy of α-glucosidase was found with Acarbose (reference compound) showed 09 amino acid residues-Asp 49, Tyr 414, Arg 417, Asn 361, Tyr 425, Asp 426, Asp 424, His 422, Gly 363 (Fig. 7); with Ambiguine I Isonitrile showed 07 amino acid residues-Leu 428, Tyr 425, Asp 426, Tyr 414, Ala 54, Val 53, Asn 50 (Fig. 8); with Scytoscalarol showed 09 amino acid residues - Asp 49, Gly 415, Tyr 414, Asp 426, Val 53, Tyr 425, Asp 424, Arg 417, Leu 428 (Fig. 9); with Cylindrospermopsin showed 11 amino acid residues - Arg 417, His 422, Asn 361, Leu 364, Val 423, Gly 415, Tyr 414, Asp 424, Tyr 425, Thr 427, Asp 426 (Fig. 10). The values of free binding energy for Acarbose with α-glucosidase; Ambiguine I Isonitrile with α-glucosidase; Scytoscalarol with α-glucosidase; Cylindrospermopsin with α-glucosidase are “–8.86, –9.46, –7.5, -7.17 kcal/mol respectively, although the conforming values of inhibition constant (Ki) were expected to be 0.31974; 0.1164; 3.20; 5.51 µM which are separately listed in Table 7.

Fig. (9). Docked structure of scytoscalarol and α-glucosidase.

Figs. (7-10): Docked structure of selected top three leads and Acarbose, a control drug, with the active site residues of α-glucosidase.

Fig. (10). Docked structure of cylindrospermopsin and α-glucosidase.
Table 7.
Active sites residues in the receptor of top three cyanobacterial bioactive compounds and metformin with Binding energy (receptor: α-glucosidase).
S. No. Name of Compounds Free Binding Energy
Kcal/mol
Interacting Residues in the Receptor
0 ACARBOSE (Reference compound) -8.86 Asp 49, Tyr 414, Arg 417, Asn 361, Tyr 425, Asp 426, Asp 424, His 422, Gly 363
1 Ambiguine I isonitriles -9.46 Leu 428, Tyr 425, Asp 426, Tyr 414, Ala 54, Val 53, Asn 50
2 Scytoscalarol -7.5 Asp 49, Gly 415, Tyr 414, Asp 426, Val 53, Tyr 425, Asp 424, Arg 417, Leu 428
3 Cylindrospermopsin -7.17 Arg 417, His 422, Asn 361, Leu 364, Val 423, Gly 415, Tyr 414, Asp 424, Tyr 425, Thr 427, Asp 426

The resultant compounds were also found to be suitable with noble druggable character, as per Lipinski’s rule of five and ADEMT profile properties. Therefore, this potent compound could be a possible drug applicant for treating type 2 diabetes mellitus [28]. The resulting cyanobacterial compounds are subjected to in-vitro analysis where phytochemical evaluation, anti-oxidant activity, anti-diabetic activity and anti-inflammatory activity. Current progress in the understanding of the biology of T2DM has resulted in an increasing number of therapies and remedies that are permitted or in clinical development for this disorder [29]. Some compounds have exhibited very interesting results and successfully reached Phase II and Phase III clinical trials [30].

CONCLUSION

Diabetes mellitus is a global metabolic disorder that is alarmingly on the rise. The prolonged use of currently available synthetic medications offers only a temporary solution and comes with undesirable side effects. The resurgence of the natural medicine field holds promise, and there is a strong call for solutions to diabetes from natural sources. Recently, there have been reports highlighting cyanobacteria as a valuable source of bio- active compounds, including Ambiguine I Isonitrile, Cylindrospermopsin, Scytoscalalrol, Ambigol A, Ambigol B, Anatoxin-a, Aplysiatoxin, Carazostatin, Carbamydo- cyclophane E, Carbamydocyclophane J, Curacin A, Curacin D, Debromoplysiatoxin, Nostocarboline, Ethyl Tumonoate A, Nostocine A, Nostocyclyne A, Radiosumin, Rutin, Scytoscalarol, Tanikolide, and Venturamide A.

Furthermore, these bioactive compounds from cyanobacteria hold immense potential in research and offer a limitless outlook for the development of new drug candidates. In this study, molecular docking analysis has unveiled that certain cyanobacterial bioactive compounds, such as Ambiguine I Isonitrile, Cylindrospermopsin, Scytoscalalrol, and Ethyl Tumonoate A, exhibit strong potential as candidates for the development of novel drugs targeting α-amylase and α-glucosidase enzymes in the treatment of type 2 diabetes mellitus. Further experimental in-vitro and in-vivo studies are needed to confirm the therapeutic effectiveness of these compounds in the development of innovative antidiabetic medications.

LIST OF ABBREVIATIONS

T2DM = Type 2 Diabetes Mellitus
DM = Diabetes Mellitus
WHO = World Health Organization

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

Not applicable.

HUMAN AND ANIMAL RIGHTS

Not applicable.

CONSENT FOR PUBLICATION

Not applicable.

AVAILABILITY OF DATA AND MATERIALS

The data and supportive information are available within the article.

FUNDING

None.

CONFLICT OF INTEREST

Salman Akhtar is the Associate Editorial Board Member of The Open Bioinformatics Journal.

ACKNOWLEDGEMENTS

The authors would like to thank Integral University, Lucknow, for providing the facilities to carry out this research work, with Manuscript Communication Number: IU/R&D/2023 MCN0002167.

REFRENCES

1
Liu L, Ma Y, Wang RL, Xu WR, Wang SQ, Chou KC. Find novel dual-agonist drugs for treating type 2 diabetes by means of cheminformatics. Drug Des Devel Ther 2013; 7: 279-88.
2
Martínez-Castelao A, Navarro-González JF, Górriz JL, De Alvaro F. The concept and the epidemiology of diabetic nephropathy have changed in recent years. J Clin Med 2015; 4(6): 1207-16.
3
Barde SR, Sakhare RS, Kanthale SB, Chandak PG, Jamkhande PG. Marine biosactive agents: A short review on new marine antidiabetic compounds. Asian Pac J Trop Dis 2015; 5: S209-13.
4
Tabatabaei-Malazy O, Larijani B, Abdollahi M. A novel management of diabetes by means of strong antioxidants’ combination. J Med Hypoth Ideas 2013; 7(1): 25-30.
5
Patel A, Chalmers J, Poulter N. ADVANCE: action in diabetes and vascular disease. J Hum Hypertens 2005; 19(1): S27-32.
6
Goldenberg R, Punthakee Z. Definition, classification and diagnosis of diabetes, prediabetes and metabolic syndrome. Can J Diabetes 2013; 37: S8-S11.
7
Groop L, Pociot F. Genetics of diabetes–are we missing the genes or the disease? Mol Cell Endocrinol 2014; 382(1): 726-39.
8
Maleki S, Falsafi P, Pakdel F, Eslami H, Ahari UZ, Pourali Baba F, et al. Comparison between catalase and salivary alpha-amylase level in patients with type I diabetes and non-diabetic people. Biomed Pharmacol J 2016; 9: 463-8.
9
Dyer J, Wood IS, Palejwala A, et al. Expression of monosaccharide transporters in intestine of diabetic humans. Am J Physiol Gastrointest Liver Physiol 2002; 282: G241-8.
10
Barrett ML, Udani JK. A proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris): a review of clinical studies on weight loss and glycemic control. Nutr J 2011; 10: 1-10.
11
Luo H, Wang LF, Imoto T, Hiji Y. Inhibitory effect and mechanism of acarbose combined with gymnemic acid on maltose absorption in rat intestine. World J Gastroenterol 2001; 7: 9-15.
12
Arif JM, Farooqui A, Siddiqui MH, Al-Karrawi M, Al-Hazmi A, Al-Sagair OA. Novel bioactive peptides from cyanobacteria: Functional, biochemical, and biomedical significance. Studies in Natural Products Chemistry 2012; 36: 111-61.
13
Paul SM, Mytelka DS, Dunwiddie CT, et al. How to improve R&D productivity: the pharmaceutical industry's grand challenge 2010.
14
Whitton BA, Potts M. Introduction to the cyanobacteria.The Ecological of Cyanobacteria 2000; 1-11.
15
Papke RT, Ramsing NB, Bateson MM, Ward DM. Geographical isolation in hot spring cyanobacteria. Environ Microbiol 2003; 5(8): 650-9.
16
Comte K, Sabacka M, Carre-Mlouka A, Elster J, Komarek J. Relationships between the Arctic and the Antarctic cyanobacteria; three Phormidium-like strains evaluated by a polyphasic approach. FEMS Microbiol Ecol 2007; 59(2): 366-76.
17
Al-Wathnani H, Johansen JR. Cyanobacteria in soils from a Mojave desert ecosystem. Monogr West N Am Nat 2011; 5: 71-89.
18
Ghasemi Y, Yazdi MT, Shafiee A, Amini M, Shokravi S, et al. Parsiguine, a novel antimicrobial substance from Fischerella ambigua. Pharm Biol 2004; 2: 318-22.
19
Dixit RB, Suseela MR. Cyanobacteria: Potential candidates for drug discovery. Anton Leeuw 2013; 103: 947-61.
20
Gademann K, Portmann C. Secondary metabolites from cyanobacteria: complex structures and powerful bioactivities. Curr Org Chem 2008; 12(4): 33-7.
21
Sivonen K, Borner T. Bioactive compounds produced by cyanobacteria. The Cyanobacteria: Molecular Biology, Genomics and Evolution.Caster Academic Press 2008; 159-97.
22
Yarchoan M, Arnold SE. Repurposing diabetes drugs for brain insulin resistance in Alzheimer disease. Diabetes 2014; 63(7): 2253-61.
23
Brogi S, Ramalho TC, Kuca K, Medina-Franco JL, Valko M. In silico methods for drug design and discovery. Front Chem 2020; 8: 612.
24
Bocanegra A, Macho-González A, Garcimartín A, Benedí J, Sánchez-Muniz FJ. Whole alga, algal extracts, and compounds as ingredients of functional foods: composition and action mechanism relationships in the prevention and treatment of type-2 diabetes mellitus. Int J Mol Sci 2021; 22(8): 3816.
25
Gupta PP, Bastikar VA, Chhajed SS. Chemical Structure Databases in Drug Discovery 2019.
26
Singh S, Gupta AK, Verma A. Molecular Properties and Bioactivity score of the Aloe vera antioxidant compounds – in order to lead finding. Res J Pharm Biol Chem Sci 2013; 4(2): 882.
27
Bonate PL, Howard DR, Eds. Pharmacokinetics in Drug Development: Regulatory and Development Paradigms.2006; 2: 132.
28
Nasab SB, Homaei A, Pletschke BI, Salinas-Salazar C, Castillo-Zacarias C, Parra Saldívar R. Marine resources effective in controlling and treating diabetes and its associated complications. Process Biochem 2020; 92: 313-42.
29
de Jesus Raposo MF, De Morais AM, De Morais RM. Bioactivity and applications of polysaccharides from marine microalgae 2014.
30
Vijayakumar S, Menakha M. Pharmaceutical applications of cyanobacteria—A review. J Acute Med 2015; 5(1): 15-23.