Bioinformatic Analysis of a “Functional Cluster” Probably Related to Retinitis Pigmentosa
Luigi Donato1, 2, 3, *, Lucia Denaro1
Identifiers and Pagination:Year: 2018
First Page: 89
Last Page: 105
Publisher Id: TOBIOIJ-11-89
Article History:Received Date: 21/12/2017
Revision Received Date: 30/3/2018
Acceptance Date: 27/4/2018
Electronic publication date: 23/05/2018
Collection year: 2018
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Retinitis pigmentosa is an eye hereditary disease caused by photoreceptor death. One of the biggest problem is represented by its genetic heterogeneity, which has not yet allowed us to found all causative genes and how known ones could influence each other, leading to retinitis etiopathogenesis.
To propose the possible relation between the “functional cluster” of vision dark adaptation, made of five phototransductional genes (RCVRN, GNB1, GNGT1, GRK7 and ARRB1), and retinitis pigmentosa onset.
A bioinformatic approach was exploited: the starting point was searching through online database as PubMed and EMBASE to acquire information about the state of art of these gene. This step was followed by an in-silico analysis, performed by softwares as Cytoscape and Genecards Suite Plus, articulated in three phases: I) identification of common pathways and genes involved in; II) collection of previously detected genes; III) deep analysis of intersected genes and implication into etiopathogenesis of analzyed disease.
The whole in-silico analysis showed that all five gene products cooperate during phototransductional activation, expecially in the dark adaptation. Interestingly, the most exciting aspect regards the direct relation with several known retinitis pigmentosa causative genes, in form of protein interactions or other pathway correlations.
Pathway analysis permitted us to hypothesize a possible role of analyzed genes in retinitis pigmentosa etiopathogenesis, also considering the key activity of their encoded proteins. Next step will be validating our hypotesis with functional assays to ensure the real meaning of this possible association, leading to new potential retinitis pigmentosa causative genes.